Leather, The first invented technology by human beings started with natural processing techniques with vegetables & earth metals, is now processed with the help of modern art of Biotechnology. Enzymes, the secreted proteins of microorganisms, work on natural proteins like collagen. The state-of-art newly generated biotechnological science being applied to depilatory action- the Un-hairing to fiber splitting- The Bating action. This bioscience also applied from Re-hydration - the soaking to Degreasing of Natural fats and grease.